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Literature summary extracted from

  • Mondal, S.; Bakthavatsalam, D.; Steimle, P.; Gassen, B.; Rivero, F.; Noegel, A.A.
    Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions (2008), J. Cell Biol., 181, 747-760.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 additional information association with the actin cytoskeleton causes a drastic increase in the autophosphorylation activity of MHCK A, which is then activated Dictyostelium discoideum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.7 cells overexpressing GFP-GEF have both unphosphorylated and autophosphorylated MHCK A Dictyostelium discoideum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.7 additional information overexpression of the RasGEF Q GEF domain activates RasB, causes enhanced recruitment of MHCK A to the cortex, and leads to cytokinesis defects in suspension phenocopying cells expressing constitutively active RasB, and myosin-null mutants, phenotype, overview. Cells overexpressing the GEF domain show at least 2fold higher levels of MHCK A associated with the cytoskeletal fractions Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 cytoskeleton MHCK A associates with the cytoskeleton and localizes to the cell cortex Dictyostelium discoideum 5856
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 130000
-
 x * 145000, phosphorylated MHCK A, SDS-PAGE, x * 130000, unphosphorylated MHCK A, SDS-PAGE Dictyostelium discoideum
2.7.11.7 145000
-
 x * 145000, phosphorylated MHCK A, SDS-PAGE, x * 130000, unphosphorylated MHCK A, SDS-PAGE Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin heavy-chain Dictyostelium discoideum role of RasGEF Q in regulating myosin II function through MHCK A, overview ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 additional information Dictyostelium discoideum MHCK A contains active RasB, and is the predominant exchange factor for RasB. RasGEF Q can bind to F-actin and has the potential to form complexes with MHCK A, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum P42527
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.7 phosphoprotein association with the actin cytoskeleton causes a drastic increase in the autophosphorylation activity of MHCK A, which is then activated Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + myosin heavy-chain role of RasGEF Q in regulating myosin II function through MHCK A, overview Dictyostelium discoideum ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin heavy-chain phosphorylation of myosin takes place at three threonine residues in the tail region by myosin II heavy chain kinases Dictyostelium discoideum ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 additional information MHCK A contains active RasB, and is the predominant exchange factor for RasB. RasGEF Q can bind to F-actin and has the potential to form complexes with MHCK A, overview Dictyostelium discoideum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 ? x * 145000, phosphorylated MHCK A, SDS-PAGE, x * 130000, unphosphorylated MHCK A, SDS-PAGE Dictyostelium discoideum

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 MHCK A
-
 Dictyostelium discoideum
2.7.11.7 myosin heavy chain kinase A
-
 Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum